A Time Course Study of Different Methods of Extraneural Scar Induction in a Rat Model

Leila Oriady Zanjani; Masoumeh Firouzi; Mohammad Hossein Nabian; Shayan Abdollah Zadegan; Mohsen Nategh; Reza Erfanian; Ahmad Mohebbi Ashtiani; Reza Shahryar Kamrani

doi:10.1055/s-0035-1571198

Journal of Reconstructive Microsurgery, Table of Contents

J Reconstr Microsurg 2016; 32(04): 309-315
DOI: 10.1055/s-0035-1571198

Original Article

Thieme Medical Publishers 333 Seventh Avenue, New York, NY 10001, USA.

A Time Course Study of Different Methods of Extraneural Scar Induction in a Rat Model

Authors

Leila Oriady Zanjani

¹Research Center for Neural Repair (RCNR), University of Tehran, Tehran, Iran

²Department of Orthopedic and Trauma Surgery, Shariati Hospital, Tehran University of Medical Sciences, Tehran, Iran
Masoumeh Firouzi

¹Research Center for Neural Repair (RCNR), University of Tehran, Tehran, Iran

³Tissue Repair Lab, Institute of Biochemistry and Biophysics (IBB), University of Tehran, Tehran, Iran
Mohammad Hossein Nabian

¹Research Center for Neural Repair (RCNR), University of Tehran, Tehran, Iran

²Department of Orthopedic and Trauma Surgery, Shariati Hospital, Tehran University of Medical Sciences, Tehran, Iran
Shayan Abdollah Zadegan

¹Research Center for Neural Repair (RCNR), University of Tehran, Tehran, Iran

³Tissue Repair Lab, Institute of Biochemistry and Biophysics (IBB), University of Tehran, Tehran, Iran
Mohsen Nategh

¹Research Center for Neural Repair (RCNR), University of Tehran, Tehran, Iran

³Tissue Repair Lab, Institute of Biochemistry and Biophysics (IBB), University of Tehran, Tehran, Iran
Reza Erfanian

¹Research Center for Neural Repair (RCNR), University of Tehran, Tehran, Iran

³Tissue Repair Lab, Institute of Biochemistry and Biophysics (IBB), University of Tehran, Tehran, Iran
Ahmad Mohebbi Ashtiani

¹Research Center for Neural Repair (RCNR), University of Tehran, Tehran, Iran
Reza Shahryar Kamrani

²Department of Orthopedic and Trauma Surgery, Shariati Hospital, Tehran University of Medical Sciences, Tehran, Iran

Abstract

Background Extraneural scar formation is a challenging problem in nerve repair. Rodent models of scar induction, with their high regenerative capacity, suffer from lack of comparable similarities with human cases. In this study, we attempted to find a reliable and reproducible method of extraneural scarring with a significant impact on the peripheral nerve function.

Methods A total of 60 rats were divided into three scar induction groups: abrasion (with a small piece of compressed steel wool), mincing (with extracorporeal mincing of adductor muscle and a 5-mm wide strip of the anterior border of the biceps femoris), and electrocoagulation (with a bipolar coagulator). Extraneural scarring was evaluated macroscopically and histologically during 8 weeks. The tibial functional index was used for behavioral analysis.

Results Among three different physical methods of scar induction that were applied, electrocoagulation had the most functional impairment (p < 0.001, two-way analysis of variance); whereas mincing produced the most adhesive and intensive scar, morphologically (p < 0.001).

Conclusion We conclude that: (1) the impact of the extraneural scar on the nerve is morphologically and functionally different, based on the method of scarring; (2) to achieve a scar model comparable to the human situation, a method in which the involved nerve is functionally impaired, is preferred over the ones that merely produce a bulky scar.

Keywords

peripheral nerve - sciatic nerve - scar - regeneration - experimental model

Full Text

References

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